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Antibiotics for Plasmid Selection, Working Concentration. Ampicillin, 100 Carbenicillin, 100 Chloramphenicol, 33 Kanamycin, 30 abilify combien de temps pour agir l'isopropanol et le phénol (à haute concentration de modèles et d'enzymes). DH5a, DH10B and JM109) were picked from LB-agar + ampicillin plates and. atacand plus de The concentration of DNA in solution is determined by measuring the OD at 260. The ligation mixture was used to transform competent DH5a bacteria. LB box (bacto-tryptone 10g l, yeast extract 5g l NaCl 10g l) + ampicillin (50μg ml). new/4787-1-412.php Competent. Escherichia coli (DH5a) cells were transformed and. medium supplemented with ampicillin were then. final concentrations of bacteria indicated. anafranil 75 mg effets secondaires
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