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AGL1 is resistant to ampicillin, so carbenicillin cannot be used to decontaminate the. Brunswick Scientific, UK), the bacterial culture is diluted 1:100 in LB medium (with. kanamycin concentration reduced to 85 µM (50 mg.l. -1. ). Plate-forme ou incubateur à basculante 1. ❑. L'utilisation d'un. Phase 1 de la purification — Concentration bactérienne et lyse. Cours 3.

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