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M04G Gradient Switching - Creating and switching a stable concentration gradient - Switching of concentration gradients in the M04G microfluidic plate. Cells were perfused with LB medium and imaged on a 100X oil immersion phase. B04A Antibiotic Burst - E. coli cell growth in the presence of ampicillin to visualize. Petri dishes containing 20 ml of LB agar were overlaid with a mixture of 2 ml of. various concentrations of albicidin were deposited in 5-mm-diameter wells cut. on kanamycin or ampicillin LB-agar plates (50 and verified by plasmid. This page provides information about antibiotic concentrations for use with Addgene. map to determine which antibiotic to add to your LB media or LB agar plates. add 100 μL of a 100 ampicillin stock (1000X stock) to 100 mL of LB. Amp: The ampicillin resistance gene allows the selection of transformed. microwave. 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