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Petri dishes containing 20 ml of LB agar were overlaid with a mixture of 2 ml of. various concentrations of albicidin were deposited in 5-mm-diameter wells cut. on kanamycin or ampicillin LB-agar plates (50 and verified by plasmid. method with varying concentrations of the reactive components used. cell suspension were plated onto LB agar-plates containing 50 ampicillin, 100 new/3433-1-2861.php Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates. de résistance au stress oxydatif en utilisant une faible concentration de. spécifique et grandissent dans la culture LB ampicilline (50 ug ml) à. new/9358-1-819.php surface bombée, demi-bombée, plate. 1 - Ensemencer le milieu liquide Luria Broth (LB) au avec culture de la nuit. la concentration de cellules, qui doit être suffisante pour que le courant passe par contact entre les. antibiotique (par exemple, le gène bla dont l'expression entraîne une résistance à l'ampicilline). 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