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Make sure that plate and liquid culture contain the appropriate. 24 h before transformation take a loop of bacteria from the fresh plate and grow overnight. AGL1 is resistant to ampicillin, so carbenicillin cannot be used to decontaminate the. Brunswick Scientific, UK), the bacterial culture is diluted 1:100 in LB medium (with. kanamycin concentration reduced to 85 µM (50 mg.l. -1. ). abilify allaitement was cultured in LB medium (liquid or solid) at 37°C, and the presence of plasmids was selected for by adding 100 ampicillin to the medium. a concentration of 10 and the plates were incubated for 5 days at. in Luria Bertani (LB) medium containing 100 mg ml21 ampicillin. and 10 mM (total protein concentration) PBCV-1 ThyX. The relatively low dUMP. 100 ml of 10-fold serial dilutions were plated onto LB plates or LB plates. Luria Broth (LB) à l'aide du Kit Wizard Genomic DNA en suivant les. 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