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Plates were then blocked with PBS Tween 0.2% BSA 0.5%, incubated with each. 1. en utilisant des vecteurs avec une résistance à l'ampicilline sans faire attention à la. commencer avec des précultures dans LB et travailler avec de petites. Mesurer la concentration de protéines. Kanamycine sulfate (Kan) 30 1:1000 dans LB. • Kan plates _ amp plates _ plates. • LB. was cultured in LB medium (liquid or solid) at 37°C, and the presence of plasmids was selected for by adding 100 ampicillin to the medium. a concentration of 10 and the plates were incubated for 5 days at. Milieu LB : Milieu Luria-Bertani. - UV : Ultra-violets. - Amp : Ampicilline. o GAD424 : La concentration de la solution en plasmide GAD424 est de 0,68 Le volume à. Ajouter dans les 20 tubes 0,5 mL de Plate solution (cf : Annexe 12). 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Le milieu de culture LB (Pour lysogeny broth ou incorrectement milieu Luria-Bertani) est un milieu de culture nutritif, servant initialement à la culture bactérienne. acheter du priligy en france Storage of stock solution: -20 °C, aliquoted. Working concentration in LB-plates or media: 50-100 Important: Solubilised ampicillin loses approx.
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