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Milieu LB : Milieu Luria-Bertani. - UV : Ultra-violets. - Amp : Ampicilline. o GAD424 : La concentration de la solution en plasmide GAD424 est de 0,68 Le volume à. Ajouter dans les 20 tubes 0,5 mL de Plate solution (cf : Annexe 12). on verse le contenu du tube dans un plus gros volume de LB + ampicilline. Après une étape de purification, on peut mesurer la concentration des. Figure 12 : les chercheurs du LMP dans le cadre de cette plate-forme de production. Par comparaison avec le LB, ce type de milieu est appauvri en métabolite. Au final, les échantillons RMN sont obtenus après concentration sur cellule Amicon. fusion est étalée sur boîte LB-agar contenant 100 d'ampicilline (Ampi). 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