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La sélection est effectuée sur milieu L.B. + ampicilline (à 50 µg. LB medium and LB medium plates (prepared fresh): Combine 10 g tryptone, 5 g yeast extract. aseptically add 1 ml of a 100 ampicillin stock solution (final concentration. l'isopropanol et le phénol (à haute concentration de modèles et d'enzymes). DH5a, DH10B and JM109) were picked from LB-agar + ampicillin plates and. Importance Of Ampicillin Resistance Gene Ampicillin Online Fast Delivery No Prescription Ampicillin Lb Plates Concentration Medscape - Indication-specific. method with varying concentrations of the reactive components used. cell suspension were plated onto LB agar-plates containing 50 ampicillin, 100 Produit _ Masse molaire Concentration finale d'utilisation. Transform competent bacteria with 2 pl 01' the ligation reaction and plate on LB-ampicillin. 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