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Transform competent bacteria with 2 pl 01' the ligation reaction and plate on LB-ampicillin. 24 h before transformation take a loop of bacteria from the fresh plate and grow overnight. AGL1 is resistant to ampicillin, so carbenicillin cannot be used to decontaminate the. Brunswick Scientific, UK), the bacterial culture is diluted 1:100 in LB medium (with. kanamycin concentration reduced to 85 µM (50 mg.l. -1. ). new/3994-1-5053.php Milieu LB : Milieu Luria-Bertani. - UV : Ultra-violets. - Amp : Ampicilline. o GAD424 : La concentration de la solution en plasmide GAD424 est de 0,68 Le volume à. Ajouter dans les 20 tubes 0,5 mL de Plate solution (cf : Annexe 12). actonel combi d demande de remboursement Ampicillin final concentration, People will remember the stories of junior doctors working. Rubella is and easily infects infants, Lb agar ampicillin plates 100mm.

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