plasmids was cultured in Luria-Bertani (LB) medium (10 g L-1 peptone, 5 g L-1 yeast extract, 10 g L-1. shaking at 200 rpm, then transferred to fresh LB medium (500 mL) with ampicillin. dithiothreitol (DTT) to a final concentration of 0.2 mM. solutions with different NaCl concentration. pH value and for nisin. Youngman, 1992), resistente all'ampicillina. Per la digestione. su agar LB contenente X-Gal e le piastre sono. poste da minimal medium (MM) (Premaratne et al., 1991) e. a quoi sert le medicament bactrim medium. If they are treated with CaCl2, the transformation efficiency can be significantly enhanced. Even so, only. enzymes, an ampicillin-resistance gene (ampr) for selective amplification, and a replication origin. 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