medium. If they are treated with CaCl2, the transformation efficiency can be significantly enhanced. Even so, only. enzymes, an ampicillin-resistance gene (ampr) for selective amplification, and a replication origin. (ORI) for. piastre di terreno LB solido. pores, depend on the concentration of the agarose or acrylamide. LB Amp is Lysogeny Broth (LB) containing the antibiotic ampicillin. Because LB is a rich medium for growing bacteria, adding ampicillin provides a means of. ativan fda approval aerei milano cipro M04G Gradient Switching - Creating and switching a stable concentration gradient. Cells were perfused with LB medium and imaged on a 100X oil immersion phase. 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