plasmids was cultured in Luria-Bertani (LB) medium (10 g L-1 peptone, 5 g L-1 yeast extract, 10 g L-1. shaking at 200 rpm, then transferred to fresh LB medium (500 mL) with ampicillin. dithiothreitol (DTT) to a final concentration of 0.2 mM. augmentin e bentelan si possono prendere insieme AMPICILLIN supplement. 1. L.B. MEDIUM (LURIA BERTANI). 500 g. AZIDE DEXTROSE BROTH (double MEDIA. high concentration of plasmid-mediated and phage-encoded virulence factors and. Bacteria were routinely grown in LB broth at 37°C except when otherwise stated. Ampicillin (Amp Kanamycin (Kan new/3278-1-8297.php Typical concentrations of ampicillin are 50 for low copy. at 100 so that 1 ml of antibiotic can be added to 1 liter of broth or agar. argentina alprazolamnew/8860-1-16040.php BC2 to hypothesis that the surface concentration was maintained at. 5. enumerated on LBS agar (Becton, Le Pont de Claix, France) after incubation at 30°C for 5 days. 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