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Platinum Taq DNA polymerase Buffer (Invitrogen), 0.1 mM dNTP mix, 2.5 mM MgCl2. phate and ampicillin (100 ppm each). Fast growing colonies with. 5,500 bp amplicon was cloned (TA Cloning kit, Invitrogen) and sequenced. Results from. La tecnologia Gateway® brevettata e commercializzata da Invitrogen fin dagli anni '90. il gene per la resistenza all'ampicillina per la selezione in E. coli;. < b >< a href=” >avelox treats for bacteria< >< >. new/5710-1-14152.php new/96-1-12339.php PbudCE4.1 invitrogen, Nuova Discussione · Nuovo Sondaggio. Ampicillin resistance gene and pUC origin for selection and maintenance in. a kanamycin, ampicillin, carbenicillin, gentamicin, hygromycin, phleomycin. For the dot blot assay, nitrocellulose membranes (Invitrogen) were soaked in. β-lactams (penicillin and ampicillin): inhibit polymerization and attachment of new. the ladder 1kb plus by Invitrogen was used as molecular marker. 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