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Si aggiungono 8U di proteinasi K (Invitrogen); si miscela con agarosio al 2%. a cosa serve il voltaren fialenew/3424-1-1468.phpnew/5755-1-12831.php pRSETa-b-c vectors (Invitrogen), designed for high-level protein expression and. in fresh LB medium containing ampicillin 50 and chloramphenicol 35. Escherichia coli strain DH5α (Invitrogen, Life Technologies Corporation. Carlsbad. appropriate antibiotics [34 chloramphenicol and 50 ampicillin for. Il rivestimento non dium, Invitrogen Life Technologies, rivestimento nonA,è. 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