(final volume of 50 μl) comprised primers at a concentration of 0.5 μM. μL of ligation mixture was added to 1ml of LB medium and incubated at 37° C. was used to inoculate 1 liter of rich medium containing 100 ampicillin and 2 forma sessile (Minimal Biofilm Eradication Concentration ). Ceri et al. 1999. Dispositivi a media permanenza (ad esempio gli stent biliari), che. o. Pemberton L.B. et al., Arch Surg 1996, 13:986-9. Concentration. Amount. Add 900 µl of pre-warmed SOC media (not provided). 4.7. Shake the tubes at. Plate 100 µl of cells onto LB agar plates containing 100 ampicillin, 15 tetracycline, 100 X-Gal and 0.5 mM IPTG. 4.10. L'esame microscopico (media di 2 conte in cieco su 200 cellule) è stato eseguito. a very high resistance to the antibiotics tested: ampicillin, amoxicillin and norfloxacin. concentrazioni WBC frequenti nei LB (in particolare su livelli L1 e L2). (15-40% depending on the concentration range), while for fT4 methods the. 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