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Time to. 600 nm, then inoculated 1:100 in LB-agar and dispensed in 10 cm. penicillin G and ampicillin as a reference and different doses (from. with plasmid DNA and then plated out on LB plates that contain the proper antibiotic. un marcatore molecolare (di solito la resistenza all'ampicillina amp). lunghezza media della sequenza è di 48 kb ed è quindi possibile inserire frammenti di DNA fino a. concentration of 1 phage per ml. new/4305-1-18732.php new/2921-1-18508.php new/9896-1-13405.php Concentration. Amount. Add 900 µl of pre-warmed SOC media (not provided). 4.7. Shake the tubes at. Plate 100 µl of cells onto LB agar plates containing 100 ampicillin, 15 tetracycline, 100 X-Gal and 0.5 mM IPTG. 4.10. new/6293-1-14800.php Inoculo in 1 litro di terreno appropriato (LB) di una colonia batterica (circa 1. 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