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1) Dissolve 1 g of ampicillin into 10 ml of ddH2O. 2) Filter through a µm filter to sterilize. 3) Aliquot and store at -20°C. 4) Use at 1:1000 dilution in LB or. The cloning vector was prepared by cutting NZYTech´s pNZY28 with EcoRV and adding a. 3´ terminal. Other protocols may be suitable but we recommend the. trasformazione - scorciatoia 5min per ampicillina plasmidi resistenti. Disclaimer: Mol Biol & Biochem Protocols is the property and trademark. Ampicillin stock solution (100 Ampicillin (sodium salt [sodium ampicillin], m.w. = 371.40). Dissolve 1 g of sodium ampicillin in sufficient H2O to make a. 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