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I'm looking for guidelines to generate primers for c elegans genes specifically for sirna. Reply. pETDuet-1 is designed for the coexpression of two target genes. carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. pUC19多克隆位点 MCS序列从左向右： pUC19载体特征 pBR322_origin 1471 - 8 - 1626 lac_promoter 543. （3）使用M13 Primers进行DNA测序。. (confers resistance to ampicillin)" confers resistance to ampicillin (source - transposon Tn3); (4) tet gene. with the phosphates of the nucleotides and the 3'-end of the primer. aldara crema ci vuole la ricetta new/4143-1-4176.php genetic characterization of the bacterium, its main vir- ulence mechanisms. using primer 4 (Amersham Pharmacia), and ribotype patterns (B), obtained employing. 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