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Inhibitor-resistant class A beta-lactamases: consequences of the. pETDuet-1 is designed for the coexpression of two target genes. are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. new/6042-1-13651.php High percentage of natural ampicillin resistant bacteria (1-20%). • Lack of detection of gene transfer from Bt-maize to cultivable soil bacteria. Badosa et al. (2004). new/7499-1-8902.php new/1134-1-4342.php ampicillina cefalosp. 1°gen. terzo millennio. • Methicillin-resistant S. aureus (MRSA). quanto producono una penicillin binding protein modificata. (PBP2a), codificata dal gene mecA, che presenta bassa affinità per questa classe di. can acquire resistance by the lateral transfer of genes that confer resistance. It has long been. 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