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Plasmids containing this hybrid gene transformed A. pullulans strain. One transformant appeared to contain four plasmids ranging in size from 4 to. A. pullulans transformant was able to transform E. coli to ampicillin resistance. ti ricercati mediante PCR i geni di patogenicità fim, invA e spvC e i geni che codificano. and antimicrobial resistance genes such as fim, invA, spvC and int 1, 2 3 and 4 were investigated by PCR. Antibiotici saggiati: Acido nalidixico (Na), ampicillina (Amp), amoxicillina+. plification of the fimA gene sequence of Salmo-. Ampicillin Resistance Enzyme Ampicillin Resistance Primers. 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