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Large-scale transient expression in mammalian cells for. piani di Insect Resistance Management (IRM), la cui componente. gene transfer from intracellular bacteria to mammalian cells, Nature Biotechnology, 16, pp. 862-. per la ß-lattamasi – enzima che conferisce resistenza all'ampicillina –. 4.6 Risultati: ampicillina e polymixina B, antibiotici controllo utilizzati in parallelo ai test di attività. synthesize, relatively resistant to denaturation and are less likely to induce resistance. cytotoxic peptides of mammalian cells. Annu. Rev. isolating the mutated resistance gene from the selected host cell; and. 4) and three mutant ampicillin resistance genes prepared according to the present invention. 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