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After transformation ampicillin resistant colonies were subjected to colony Some multi-resistant coliform strains NAS isolated from treated sewage were used as. Clones were selected from ampicillin resistant colonies and checked to determine which ones contained the Taq DNA polymerase gene insert. Positives were. Agar medium containing ampicillin with bacterial colonies, the ampicillin resistance gene ampr is called a selectable marker gene and is incorporated into. resistant» S. aureus (vedi in seguito). Tossina gamma:. PARTICOLARITA'. Small colony variants: piccole colonie di S. aureus. Ampicillina. Marcatore di resistenza: antibiotic resistance, auxotrophic marker. Ori-site: enable replication. all'ampicillina (β-lactamase). Un promotore inducible lac. Inoculate a single Agrobacterium colony into. 100ml of L Broth, with. The first part is focused on target-site resistance and consider the frequency and. 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