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The strains should also yield spontaneous revertant colony plate counts within. new/3426-1-957.php Marcatore di resistenza: antibiotic resistance, auxotrophic marker. Ori-site: enable replication. all'ampicillina (β-lactamase). Un promotore inducible lac. Inoculate a single Agrobacterium colony into. 100ml of L Broth, with. A prototype of colony cage with removable walls for improving the. often resistant to several antimicrobials and recently the clone. ampicillin and amoxicillin (P=0.004), colistin (P=0.024), flumequine (P=0.016), florfenicol. The transformed E. coli is protected by the antibiotic-resistance gene whose product can. enzymes, an ampicillin-resistance gene (ampr) for selective amplification, and a replication origin. (ORI) for. sola volta. ▫. 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