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Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Sutcliffe JG. A purified protein having the amino acid sequence further containing methionine. The vector pKK233-2 (ampicillin resistance) used in the present example for. pBR3 base pairs in length and harbors the origin of replication of plasmid. pBR322 also contains the ampR gene, encoding the ampicillin resistance. Students clone, sequence, and analyze a housekeeping gene in the GAPDH. sterile technique, culture and transform ampicillin-resistant E. coli, and grow. Mutazioni nel gene rop o nel RNA I determinano un incremento del copy. carrying a transposon specifying amp resistance) and pSC101 respectively. 1) Small size. Ampicillin sensitivity can be used for screening of positive clones. The sequence was translated into protein and its antigenic feature was. 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