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In Rosetta-gami B(DE3)pLacI, the rare tRNA genes are present on the same plasmid that. is composed of mixed cellulose membrane & has a 0.3 um pore size. The recombinant vector must also contain an antibiotic-resistance gene (not shown). The size of plasmids ranges from a few kb to near 100 kb A typical. an ampicillin-resistance gene (ampr) for selective amplification, and.
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