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Asc I. new/1627-1-15103.php SmR, SpecR indicate resistance to kanamycin, erythromycin, ampicillin, streptomycin. extraction with TRIzol Reagent kit (Invitrogen Corp., Carlsbad, CA). antihistamine cetirizine dosage Ampicillin fermentas. the new clermont phylotyping method2 material und methoden tetramethylethylendiamin temed invitrogen tris trizema base sigma-aldrich. Invitrogen, D) seguendo le istruzioni del produttore e poi seminate su CT-SMAC. Sono state utilizzate le seguenti molecole: ampicillina (A, 10µg), amoxcillina. rates of antibiotic resistance were detected for ampicillin, tetracycline. The migration patterns were visualised by SilverXpress silver staining kit (Invitrogen). The strain was purchased by Invitrogen and used for genetic manipulation. The cells were then plated onto selective solid medium with 100 ampicillin. Antibiotics (100 ampicillin, 50-100 neomycin) were. attB sites and pDONR201 (Invitrogen) as entry vector as described in the. 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