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Incubazione. sealed bag containing high carbon dioxide concentrations and low oxygen. supplement Ampicillin and Vancomycin partly inhibits growth of acompanying. were grown at 30°C in LB medium supplemented with 100 ampicillin, 1 mM. imidazole was added to the supernatant to a final concentration of 10 mM. BC2 to hypothesis that the surface concentration was maintained at. 5. enumerated on LBS agar (Becton, Le Pont de Claix, France) after incubation at 30°C for 5 days. G (Pen G), penicillin V (Pen V), and ampicillin. M04G Gradient Switching - Creating and switching a stable concentration gradient. Cells were perfused with LB medium and imaged on a 100X oil immersion phase. B04A Antibiotic Burst - E. coli cell growth in the presence of ampicillin to. In queste settimane si sta riscrivendo la geografia politica europea ed italiana. In Francia si ? chiuso un lungo ciclo neoconservatore guidato. 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