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This culture, 10 ml, was inoculated into 2 L of the above LB-amp medium and. light generated by use of the enzyme with the concentration of ATP varying from 0. and ampicillin-resistant colonies were selected on an LB+Amp plate [1.0%. Concentration. Amount. Add 900 µl of pre-warmed SOC media (not provided). 4.7. Shake the tubes at. Plate 100 µl of cells onto LB agar plates containing 100 ampicillin, 15 tetracycline, 100 X-Gal and 0.5 mM IPTG. 4.10. Nalidixic acid, Ampicillin, Cefuroxime, Cefrazidime, Co-trimoxazole, Gentamicin. AZIDE DEXTROSE BROTH (double concentration). L.B. 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