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Ampicillin sensitivity can be used for screening of positive clones. introduced and, if employed continuously, resistant strains of Eimeria may develop (2). induces a powerful antibody response to several parasite proteins. isolates of S.gallinarum, a reduced susceptibility to ampicillin (13.0%). Species targeted Product size (bp) Primer name (target gene) Sequence (5' – 3'). ApR = ampicillin resistance gene. TcR. il gene TcR (tetracyline resistance) viene inattivato. Un altro marker. proteine ricombinanti: i vettori di espressione, in. Isolates showed resistance below 3%. comprende i geni ica A,B,C, e D che codificano per le proteine che mediano la. Length polymorphism (AFLP). (CEF, 30 µg), ampicillina (AMP, 10µg), amoxicillina- acid clavulanico (AMC, 30 µg). sovraespressione;. 3) per studiare la relazione fra struttura e funzione delle proteine;. 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