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Ampicillin (50 6 x 1.5 mL. Concentration. Amount. Add 900 µl of pre-warmed SOC media (not provided). 4.7. Shake the tubes at. Plate 100 µl of cells onto LB agar plates containing 100 ampicillin, 15 tetracycline, 100 X-Gal and 0.5 mM IPTG. 4.10. you have to make LB (Luria Broth) ampicillin plates. the final ampicillin concentration should be100 micro in the media. you make 1L. Typically, filter-sterile ampicillin (Amp) stock solutions were directly diluted in LB media up to a final concentration of 100 Plasmid. 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