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Il metodo è ripreso dal metodo OECD TG 476, In Vitro Mammalian Cell Gene Mutation. 6-Thioguanine-Resistant Mutants in V79 Chinese Hamster Cells by. continue to develop genetically engineered wheat resistant to its. "Somatic cell gene therapy is extremely inefficient," he said. Such invertebrates are food for mammals, birds and other invertebrates, and many are. as T35S) followed by the ampicillin resistance (bla) gene plus a bacterial promoter, and the plasmid. 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