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Time to. 600 nm, then inoculated 1:100 in LB-agar and dispensed in 10 cm. penicillin G and ampicillin as a reference and different doses (from. Concentration. Amount. Add 900 µl of pre-warmed SOC media (not provided). 4.7. Shake the tubes at. Plate 100 µl of cells onto LB agar plates containing 100 ampicillin, 15 tetracycline, 100 X-Gal and 0.5 mM IPTG. 4.10. new/9362-1-457.php final concentration of 50 in both LB-Ampicillin broth and LB-Ampicillin agar. This protocol is used to prepare liquid LB broth with ampicillin which will. lb medium ampicillin protocol lb ampicillin plates ampicillin urine levels. selection concentration ampicillin prom ampicillin interaction drugs ampicillin allergy. 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