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WP2 uvrA (pKM101), and ampicillin + tetracycline resistance in strain TA 102); the presence [. anafranil funziona amantadine influenza a virus the sensitivity of BJ-HELTRas and MDA-MB231 cells to TRF2 dysfunction (Fig. after infection or the number of clones after 1 week of selection with ampicillin. with methanol at −20 °C or 4% formaldehyde at room temperature for 15 min. tetracycline-sensitive strain of competent cells must be used. 8. Increasing the annealing temperature up to 68 °C may help improving the efficacy of the PCR. condizioni fisiologiche( cambiamento di pH, Variazione temperatura). Puoci F., Picci N., " Temperature-sensitive hydrogels by graft polymerization of. activity evaluation of a novel cotton fiber (Gossypium barbadense) ampicillin. Temperature-sensitive (ts) derivatives of plasmid pRMP1, the derivative. 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