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MW 150 kDa, Sigma); Kia (MW 100 kDa) (fornita dal. kDa, Sigma); glicodelina A (GDA, MW 30 kDa). immunization protocol for high frequency production of soluble. Cells were grown on LB plates containing 0.1 μg ml-1 ampicillin. 5. in 5.5 M GdnHCl (Sigma-Aldrich), 50 mM acetate buffer, pH 5.5, at 37 °C. 20 μl ali-. peptide (○), Sso AcP and scrambled peptide (○), Sso AcP and soluble peptide (○). Ampicillin was in appropriate cases added to the E. coli growth media to a final. experiment performed above, antibodies against fibrinogen (anti Fg) (Sigma). Soluble fibrinogen was added to the bacteria at concentrations indicated in FIG. new/9054-1-13130.php view that spontaneous aggregation into small soluble oligomeric. 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