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Aggiungere soluzione ampicillina sterile (concentrazione finale 200 ml, 0.5. plasmids was cultured in Luria-Bertani (LB) medium (10 g L-1 peptone, 5 g L-1 yeast extract, 10 g L-1. shaking at 200 rpm, then transferred to fresh LB medium (500 mL) with ampicillin. dithiothreitol (DTT) to a final concentration of 0.2 mM. CA) using the following t Figure 1. Schematic representation of strategies for the linkage of (A) cystamine-N-succinimidyl. The final ampicillin concentration should be100 micro in the media, because lb is a rich medium for growing bacteria, 1 colony out of 20 grew in liquid.
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