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Trattamento con fenolo fenolo-cloroformio per allontanare le proteine. carrying a transposon specifying amp resistance) and pSC101 respectively. 1) Small size. Ampicillin sensitivity can be used for screening of positive clones. Ampicillin resistant; -galactosidase negative (White on X-Gal). LacZ gene. Insert size. Equal. Correlate with. expression level. Representation. Only one. Expression vs. protein. Probe. Gene structure. Infer protein identity. Encoded protein.
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