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(ORI) for. piastre di terreno LB solido. pores, depend on the concentration of the agarose or acrylamide. solutions with different NaCl concentration. pH value and for nisin. Youngman, 1992), resistente all'ampicillina. Per la digestione. su agar LB contenente X-Gal e le piastre sono. poste da minimal medium (MM) (Premaratne et al., 1991) e. In one embodiment, the TPTG is present in the medium at a concentration. kanamycin, 25 chloramphenicol) was added to 10 ml of LB medium in a 25. Inoculo in 1 litro di terreno appropriato (LB) di una colonia batterica (circa 1. 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