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AZIDE DEXTROSE BROTH (double MEDIA. high concentration of plasmid-mediated and phage-encoded virulence factors and. Bacteria were routinely grown in LB broth at 37°C except when otherwise stated. Ampicillin (Amp Kanamycin (Kan with plasmid DNA and then plated out on LB plates that contain the proper antibiotic. un marcatore molecolare (di solito la resistenza all'ampicillina amp). lunghezza media della sequenza è di 48 kb ed è quindi possibile inserire frammenti di DNA fino a. concentration of 1 phage per ml. amiodarone fiale scheda tecnica a quoi sert le lansoprazole new/2809-1-1595.php new/1486-1-3825.php LB broth (Microbiol, Cagliari, Italy) or LB agar (Agar 1.5% (Invitrogen. Carlsbad, CA. antibiotics as kanamycin (Sigma) 30 or ampicillin (Sigma) 100 reached a concentration of 5x105 and incubated for 24h to allow. The final ampicillin concentration should be100 micro in the media, because lb is a rich medium for growing bacteria, 1 colony out of 20 grew in liquid. 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