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Bacterial cultures were grown in LB broth in the presence of ampicillin at 100 For stable expression, the cells were treated with 50 Zeocin and the. et al., 1989) or in low salt LB medium NaCl), in the presence of Zeocin antibiotic. of DH5α strain was carried out with the Ampicillin or Zeocin. integrazione del GOI Psv40 ATG FRT lacZ-Zeocin Amp r pUC ori. origin - bla promoter - Ampicillin (bla) resistance gene (ß- lactamase). new/9690-10-2021.php

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